TransNGS Library Quantification DNA Standards (S1-S6) – KS101

DNA Standards for NGS Library Quantification

TransNGS™ Library DNA Quantification Standards (S1-S6) is DNA Standard used to quantify DNA libraries prepared for Illumina next generation sequencing platforms. Five linear DNA standards with different GC contents (25%, 37.5%, 50%, 62.5%, 75%) are provided for customers to choose from. All Standards come with six 10-fold dilutions (S1-S6) with the concentrations from 0.0002 pM to 20 pM. The amplified product size is 420 bp.

The DNA standards are compatible with libraries and PCR primers which target the P5 and P7 adaptor sequences. TransNGS Library Quantification qPCR SuperMix (2x) or TransNGS™ Library Quantification Kit for Illumina® or any other vendor kit are compatible.

Features of NGS DNA Standards (S1-S6) – KS101

• DNA standards with different GC content : Five sets of linear DNA Standards (25%, 37.5%, 50%, 62.5%, and 75% of GC contents) to choose from.
• Pre-dilution : Each DNA Standard contains six 10-fold dilution series of a linear 420 bp template with the concentrations from 0.0002 pM to 20 pM.

Contents of the TransNGS Library Quantification DNA Standards (S1-S6) – KS101

Table1 DNA Standard Concentration Copy Number

Procedure for NGS Library Quantification using KQ101, KQ201, KS101

Example of NGS Library Quantification

Two DNA libraries were prepared from 50 ng of Arabidopsis thaliana genomic DNA and 50 ng of Escherichia coli genomic DNA using TransNGS Tn5 DNA Library Prep Kit for Illumina®. The average fragment length and concentration of these 2 libraries were measured with Agilent Bioanalyzer (see table below).
An initial 1/10,000 dilution and one additional 2-fold (i.e. 1/20,000) dilution of these two libraries was prepared. Because the GC content of genomic DNA of Arabidopsis thaliana is about 36%, DNA Standard with 37.5% of GC contents was used to measure the library concentration. And the GC content of Escherichia coli is about 50%, DNA Standard with 50% of GC contents was used for Escherichia Coli library. The results and the calculated data are shown in the table below.

Manual & Datasheet

KS101 TransNGS Library Quantification DNA Standards (S1-S6)