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MagicPure DNA Size Selection Beads – EC401

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MagicPure DNA Size Selection Beads

MagicPure™ DNA Size Selection beads is based on the technology of solid phase reverse immobilization. The beads are suitable for purify, concentrate, and size selection of DNA when constructing NGS library. MagicPure™ Size Selection DNA beads can be used together with any library preparation kits.


Components provided with MagicPure DNA Size Selection Beads

Magnetic DNA Beads
EC401-01: 1 ml
EC401-02: 5 ml
EC401-03: 60 ml


Procedure for Size Selection of DNA

When preparing WGS (Whole genome sequencing) library, you can use the DNA beads according the conditions bellow directly prior to end repair of fragmented DNA or after library amplification. If you want to size select DNA after adapter ligation, it is preferable to purify your sample with 1.0x DNA beads before performing size selection DNA.

Reagents to be  supplied by users: freshly prepared 80% ethanol
Use at room temperature.


Purification of DNA

Choose the ratio of DNA beads before purification according to the following figure.

Agilent high sensitivity DNA chip electropherogram

Smear – Input control DNA sample in water; 0.6×~1.8× – purified DNA samples with given ratio of DNA beads volume to sample volume.

 

Agilent high sensitivity DNA chip electropherogram 2


Reference conditions for DNA size selection

Reference conditions for DNA size selection

Agilent DNA 1000 chip electropherogram
Smear – Input control DNA sample in water; 1.0+0.2~0.5+0.15 – size selected DNA samples with given conditions for size selection.


Manual & Datasheet

EC401 MagicPure DNA Size Selection Beads

Additional information

Format

1 ml, 5 ml, 60 ml, 450 ml

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