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TransNGS Tn5 Index Kit for Illumina® – KI101

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TransNGS Tn5 Indexing Kit for Illumina®

TransNGS™ Tn5 Index Kit for Illumina® contains primers that are ideally suited for multiplex sample preparation for next-generation sequencing. This kit contains 12 unique Index 1 (i7) Primers (N701-N712) and 8 unique Index 2 (i5) Primers (N501-N508) at the concentration of 10 μM. 96 single dual-indexed samples can be prepared using this kit.

The TransNGS™ Tn5 Indexing Kit for Illumina® is compatible with other TransNGS series products.

The Tn5 Index kit is especially well suited to be used with the TransNGS™ Tn5 DNA Library Prep Kit for Illumina®, (KP101). It allows to create up to 96 uniquely tagged sequencing libraries.


Content of the TransNGS Tn5 index kit for Illumina®

Content of the TransNGS Tn5 index kit for Illumina


Tn5 Index Primer Information

Index primer information

S7: Complementary pairing sequence of transposition product; Partial sequence of Read 2 Sequencing Primer;
S5: Complementary pairing sequence of transposition product; Partial sequence of Read 1 Sequencing Primer.


General Information about Tn5 indexing

G/T is detected by a green laser and A/C is detected by a red laser when using Illumina sequencer (Table 1). Color balance is required during the process of sequencing. It is necessary to make sure that both green laser bases and red laser bases are included at each cycle when combining indexes. Some of correct and incorrect combinations are given in table 2. Libraries pooling guidance is provided in table 3.

Table 1 Index Sequences

Table 2 Examples of Correct and Incorrect Index Combinations

Table 3 Library Pooling Guide


Manual & Datasheet

KI101 TransNGS Tn5 Index Kit for Illumina

Additional information

Format

96 indices for 48 rx, 96 indices for 192 rx

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