Rat VF (Visfatin) ELISA Kit – E-EL-R1067

Rat VF (Visfatin) ELISA Kit

The VF ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to visfatin. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for visfatin and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain visfatin, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of visfatin. You can calculate the concentration of visfatin in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Visfatin

Abbreviation : VF

Uniprot ID : Q80Z29

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

More information about this Rat VF (Visfatin) ELISA Kit from Elabscience