Rat TSH (Thyroid Stimulating Hormone) ELISA Kit – E-EL-R0976

Rat TSH (Thyroid Stimulating Hormone) ELISA Kit

The TSH ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TSH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TSH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TSH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TSH. You can calculate the concentration of TSH in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Thyroid Stimulating Hormone

Abbreviation : TSH

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.75 ng/ml

Detection Range : 1.25 – 80 ng/ml

Manual & Datasheet

More information about this Rat TSH (Thyroid Stimulating Hormone) ELISA Kit from Elabscience