Rat TP53 (Tumor Protein 53) ELISA Kit – E-EL-R1042

Rat TP53 (Tumor Protein 53) ELISA Kit

The TP53 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TP53. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TP53 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TP53, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TP53. You can calculate the concentration of TP53 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Tumor Protein 53

Abbreviation : TP53

Uniprot ID : P10361

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

More information about this Rat TP53 (Tumor Protein 53) ELISA Kit from Elabscience