Rat TGF-β1 (Transforming Growth Factor β1) ELISA Kit – E-EL-R0084

Rat TGF-β1 (Transforming Growth Factor β1) ELISA Kit

The TGF-β1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TGF-β1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TGF-β1. You can calculate the concentration of TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Transforming Growth Factor β1

Abbreviation : TGF-β1

Uniprot ID : P17246

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Rat TGF-β1 (Transforming Growth Factor β1) ELISA Kit from Elabscience