Rat TFF2 (Trefoil Factor 2) ELISA Kit
The TFF2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TFF 2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TFF 2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TFF 2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TFF 2. You can calculate the concentration of TFF 2 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Trefoil Factor 2
Abbreviation : TFF2
Uniprot ID : Q09030
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 37.5 pg/ml
Detection Range : 62.5 – 4000 pg/ml
More information about this Rat TFF2 (Trefoil Factor 2) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
---|
Ask a question about Rat TFF2 (Trefoil Factor 2) ELISA Kit – E-EL-R1023
You must be logged in to post a review.
Reviews