Rat sPECAM-1 (Soluble Platelet Endothelial Cell Adhesion Molecule 1) ELISA Kit – E-EL-R0902

Rat sPECAM-1 (Soluble Platelet Endothelial Cell Adhesion Molecule 1) ELISA Kit

The sPECAM-1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sPECAM-1/sCD31. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat sPECAM-1/sCD31 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sPECAM-1/sCD31, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat sPECAM-1/sCD31. You can calculate the concentration of Rat sPECAM-1/sCD31 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Soluble Platelet Endothelial Cell Adhesion Molecule 1

Abbreviation : sPECAM-1

Uniprot ID : Q3SWT0

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.094 ng/ml

Detection Range : 0.156 – 10 ng/ml

Manual & Datasheet

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