Rat BMF (BCL-2 Modifying Factor) ELISA Kit – E-EL-R0099

Rat BMF (BCL-2 Modifying Factor) ELISA Kit

The BMF ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMF. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat BMF and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMF, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BMF. You can calculate the concentration of Rat BMF in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat BCL-2 Modifying Factor

Abbreviation : BMF

Uniprot ID : Q8K589

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

More information about this Rat BMF (BCL-2 Modifying Factor) ELISA Kit from Elabscience