Rat BK (Bradykinin) ELISA Kit – E-EL-R0124

Rat BK (Bradykinin) ELISA Kit

The BK ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with BK. During the reaction, Rat BK in the sample or standard competes with a fixed amount of BK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat BK. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat BK in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Rat Bradykinin

Abbreviation : BK

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Competitive

Sensitivity : 0.75 ng/ml

Detection Range : 1.25 – 80 ng/ml

Manual & Datasheet

More information about this Rat BK (Bradykinin) ELISA Kit from Elabscience