Rat AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit – E-EL-R0738

Rat AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

The AMPK ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AMPK. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for AMPK and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AMPK, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of AMPK. You can calculate the concentration of AMPK in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Phosphorylated Adenosine Monophosphate Activated Protein Kinase

Abbreviation : AMPK

Uniprot ID : Q09137

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 1.875 ng/ml

Detection Range : 3.125 – 200 ng/ml

Manual & Datasheet

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