Mouse GRP78 (Glucose Regulated Protein 78) ELISA Kit
The ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GRP78. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse GRP78 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GRP78, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse GRP78. You can calculate the concentration of Mouse GRP78 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Mouse Glucose Regulated Protein 78
Abbreviation : GRP78
Uniprot ID : P20029
Format : 96 rx
Reactivity : Mouse
ELISA configuration : Sandwich
Sensitivity : 0.188 ng/ml
Detection Range : 0.313 – 20 ng/ml
More information about this Mouse GRP78 (Glucose Regulated Protein 78) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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Reactivity | Mouse |
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