Mouse TWIST1 (Twist-related protein 1) ELISA Kit – E-EL-M2690

Mouse TWIST1 (Twist-related protein 1) ELISA Kit

The ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TWIST1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse TWIST1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TWIST1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TWIST1. You can calculate the concentration of Mouse TWIST1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse Twist-related protein 1

Abbreviation : TWIST1

Uniprot ID : P26687

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 18.75 pg/ml

Detection Range : 31.25 – 2000 pg/ml

Manual & Datasheet

More information about this Mouse TWIST1 (Twist-related protein 1) ELISA Kit from Elabscience