Mouse HPF4 (Heparin/Platelet Factor 4 Antibodies) ELISA Kit – E-EL-M2694

Mouse HPF4 (Heparin/Platelet Factor 4 Antibodies) ELISA Kit

The ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HPF4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse HPF4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HPF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse HPF4. You can calculate the concentration of Mouse HPF4 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Mouse anti-Heparin/Platelet Factor 4

Abbreviation : HPF4-Ab

Uniprot ID :

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Mouse HPF4 (Heparin/Platelet Factor 4 Antibodies) ELISA Kit from Elabscience