Mouse 4-HNE (4-Hydroxynonenal) ELISA Kit – E-EL-M2677

Mouse 4-HNE (4-Hydroxynonenal) ELISA Kit

The ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Mouse 4-HNE. During the reaction, Mouse 4-HNE in the sample or standard competes with a fixed amount of Mouse 4-HNE on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to Mouse 4-HNE. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Mouse 4-Hydroxynonenal

Abbreviation : 4-HNE

Uniprot ID :

Format : 96 rx

Reactivity : Mouse

ELISA configuration : Competitive

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

More information about this Mouse 4-HNE (4-Hydroxynonenal) ELISA Kit from Elabscience