Neurofilament NF-M Antibody – MCA-3H11

Encor Mouse monoclonal to Neurofilament Medium, NF-M

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries.

Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig’s disease) and Alzheimer’s disease.

MCA-3H11 was raised against a recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli (1). The epitope is localized to within the last 56 amino acids at the extreme C-terminus of rat NF-M, the so-called KE segment which is highly conserved between NF-M molecules from different species. MCA-3H11 is an IgG1 class antibody with a k light chain. It recognizes intact NF-M strongly and specifically on immunoblots in all mammals and birds tested to date and also binds NF-M in cells in tissue culture and in sectioned material.

HGNC name(s) : NEFM

Host : Mouse

Clonality : Monoclonal

ID : EnCor Biotechnology Neurofilament NF-M 3H11

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG1

Conjugation : none

Immunogen : Recombinant fusion protein with extreme C-term rat NF-M

Mass of detected protein : 145 kDa by SDS-PAGE

Uniprot ID : P07197

KGNC name : NEFM

RRID # : AB_2572365

Purification : Ascites

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:5 000. IF/ICC and IHC: 1:500.

Datasheet

Link to on EnCor’s website

References :

1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.