Neurofilament NF-H Antibody – MCA-9B12
- Clonality : Mouse Monoclonal
- Applications : WB | IF/ICC | IHC
- Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse
299.00$ – 1,597.00$ CAD
Encor Mouse Monoclonal to Neurofilament NF-H
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries.
Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig’s disease) and Alzheimer’s disease.
MCA-9B12 is a mouse monoclonal antibody raised against a preparation of native NF-H purified from bovine spinal cord. MCA-9B12 is an IgG2b class antibody with a k light chain. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.
HGNC name(s) : NEFH
Host : Mouse
Clonality : Monoclonal
ID : EnCor Biotechnology Neurofilament NF-H 9B12
Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse
Isotype : IgG2b
Conjugation : none
Immunogen : Purified bovine
Mass of detected protein : 200 kDa
Uniprot ID : P12036
KGNC name : NEFH
RRID # : AB_2572358
Purification : Ascites
Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.
Validated applications : WB | IF/ICC | IHC
WB: 1:10 000. ICC/IF and IHC: 1:1 000.
1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.
50 ul, 100 ul, 500 ul
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Product Reviews for Neurofilament NF-H Antibody – MCA-9B12
299.00$ – 1,597.00$ CAD