Neurofilament NF-H Antibody – MCA-9B12

Encor Mouse Monoclonal to Neurofilament NF-H

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries.
Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (also known as Lou Gehrig’s disease) and Alzheimer’s disease.

MCA-9B12 is a mouse monoclonal antibody raised against a preparation of native NF-H purified from bovine spinal cord. MCA-9B12 is an IgG2b class antibody with a k light chain. It recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections.

HGNC name(s) : NEFH

Host : Mouse

Clonality : Monoclonal

ID : EnCor Biotechnology Neurofilament NF-H 9B12

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgG2b

Conjugation : none

Immunogen : Purified bovine

Mass of detected protein : 200 kDa

Uniprot ID : P12036

KGNC name : NEFH

RRID # : AB_2572358

Purification : Ascites

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:10 000. ICC/IF and IHC: 1:1 000.

Datasheet

Link to on EnCor’s website

References :

1. Harris, J., Ayyub, C. and Shaw G. A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62 1991.