Visinin-like protein 1 Antibody – CPCA-VLP1

Encor Chicken Polyclonal to Visinin-like Protein 1

Visinin was originally isolated biochemically from chicken retina as a major protein of ~24 kDa on SDS-PAGE (1). Following cloning and sequencing of visinin, several visinin like proteins were discovered by homology screening (2, 3). One of these, Visinin-like protein 1 is a small Calcium binding protein which is very abundant in the nervous system and is found only in neurons, though different neurons have different levels of expression (4, 5). It is particularly concentrated in cerebellar Purkinje cells, and tends to be most abundant in perikarya and dendrites.
The protein was discovered independently by several groups and is therefore also sometimes known as hippocalcin-like protein 3, HLP3, HPCAL3, HUVISL1, VLP-1, VILIP and VILIP-1The protein belongs to the large superfamily of calmodulin and paravalbumin type proteins which function by binding Calcium ions. Calcium binding alters the conformation of these proteins and allow them to interact with other binding partners, the properties of which they may alter. Visinin-like protein 1 has four “EF hand” domains, which are negatively charged helix-turn-helix peptides which are responsible for Calcium binding.

Visinin-like protein 1 is 191 amino acids in size and has a molecular weight on SDS-PAGE of 22 kDa. The protein has recently been suggested to be a useful biomarker of Alzheimer’s disease and traumatic brain injury (6, 7, 8). The HGNC name for this protein is VSNL1.

Antibody was raised in chicken against recombinant full length His-tagged VLP-1 purified from E. coli. and is known to react with VLP-1 from human, cow, mouse and rat. Since VLP-1 is highly conserved in primary sequence, it is likely that the antibody is effective on other species also

HGNC name(s) : VSNL1

Host : Chicken

Clonality : Polyclonal

ID : EnCor Biotechnology Visinin-like protein 1 VLP1

Reactivity : Human | Horse | Cow | Pig | Chicken | Rat | Mouse

Isotype : IgY

Conjugation : none

Immunogen : Recombinant full length human

Mass of detected protein : 22 kDa

Uniprot ID : P62760

KGNC name : VSNL1

RRID # : AB_2572401

Purification : Concentrated IgY preparation

Storage : Shipped on ice. Store at 4°C. For long term storage, leave frozen at -20°C. Avoid freeze / thaw cycles.

Validated applications : WB | IF/ICC | IHC

Suggested Dilutions:

WB: 1:5 000-10 000. IF/IHC: 1:1 000-2 000

Datasheet

Link to on EnCor’s website

References :

1. Hatakenaka S, Kuo CH, Miki N. Analysis of a distinctive protein in chick retina during development. Brain Res. 312:155-63 (1983).

2. Kuno T, Kajimoto Y, Hashimoto T, Mukai H, Shirai Y, Saheki S, Tanaka C. cDNA cloning of a neural visinin-like Ca(2+)-binding protein. Biochem Biophys Res Commun. 184:1219-25 (1992).

3. Polymeropoulos MH, Ide S, Soares MB, Lennon GG. Sequence characterization and genetic mapping of the human VSNL1 gene, a homologue of the rat visinin-like peptide RNVP1. Genomics. 29:273-5 (1995).

4. Bernstein HG, Baumann B, Danos P, Diekmann S, Bogerts B, Gundelfinger ED, Braunewell KH. Regional and cellular distribution of neural visinin-like protein immunoreactivities (VILIP-1 and VILIP-3) in human brain. J Neurocytol. 28:655-62 (1999).

5. Paterlini M, Revilla V, Grant AL, Wisden W Expression of the neuronal calcium sensor protein family in the rat brain.
.Neuroscience. 99:205-16 (2000).

6. Laterza OF, Modur VR, Crimmins DL, Olander JV, Landt Y, Lee JM, Ladenson JH. Identification of novel brain biomarkers. Clin. Chem. 9:1713-21 (2006).

7. Lee JM, Blennow K, Andreasen N, Laterza O, Modur V, Olander J, Gao F, Ohlendorf M, Ladenson JH. The brain injury biomarker VLP-1 is increased in the cerebrospinal fluid of Alzheimer disease patients. Clin. Chem. 10:1617-23 (2008).

8. Tarawneh R, D’Angelo G, Macy E, Xiong C, Carter D, Cairns NJ, Fagan AM, Head D, Mintun MA, Ladenson JH, Lee JM, Morris JC, Holtzman DM. Visinin-like protein-1: diagnostic and prognostic biomarker in Alzheimer disease. Ann Neurol. 70:274-85 (2011) doi: 10.1002/ana.22448.