VK1 (Vitamin K1) ELISA Kit – E-EL-0019

VK1 (Vitamin K1) ELISA Kit

The VK1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VK1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for VK1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VK1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VK1. You can calculate the concentration of VK1 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: VK1

Abbreviation : Vitamin K1, phytomenadione, phytonadione

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 9,375

Detection Range : 15.625-1000 pg/mL

More information about this VK1 (Vitamin K1) ELISA Kit from Elabscience