LTB4 (Leukotriene B4) ELISA Kit – E-EL-0061

LTB4 (Leukotriene B4) ELISA Kit

The LTB4 ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in the sample or standard competes with a fixed amount of LTB4 on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to LTB4. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of LTB4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: LTB4

Abbreviation : Leukotriene B4

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 9,375

Detection Range : 15.625-1000 pg/mL

More information about this LTB4 (Leukotriene B4) ELISA Kit from Elabscience