Cotinine ELISA Kit – E-EL-0064

Cotinine ELISA Kit

The Cotinine ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with Cotinine. During the reaction,  Cotinine in the sample or standard competes with a fixed amount of Cotinine on the solid phase supporter for sites on the Biotinylated Detection Ab specific to  Cotinine. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of  Cotinine in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: Cotinine

Abbreviation :

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,375

Detection Range : 0.625-40 ng/mL

More information about this Cotinine ELISA Kit from Elabscience