DHT (Dihydrotestosterone) ELISA Kit - E-EL-0031

DHT (Dihydrotestosterone) ELISA Kit

The DHT ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with DHT. During the reaction, Universal DHT in the sample or standard competes with a fixed amount of DHT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal DHT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal DHT in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: DHT

Abbreviation : 5α-DHT, Androstanolone, 5α-androstan-17β-ol-3-one, 17β-hydroxy-5α-androstan-3-one

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 23,438

Detection Range : 39.063-2500 pg/mL

More information about this DHT (Dihydrotestosterone) ELISA Kit from Elabscience