17-OHP (17-Hydroxyprogesterone) ELISA Kit – E-EL-0039

17-OHP (17-Hydroxyprogesterone) ELISA Kit

The 17-OHP ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with 17-OHP. During the reaction, Universal 17-OHP in the sample or standard competes with a fixed amount of 17-OHP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 17-OHP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal 17-OHP in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: 17-OHP

Abbreviation : 17OHP, 17-OH Progesterone

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,188

Detection Range : 0.313-20 ng/mL

More information about this 17-OHP (17-Hydroxyprogesterone) ELISA Kit from Elabscience