NA/NE (Noradrenaline/Norepinephrine) ELISA Kit – E-EL-0047
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NA/NE (Noradrenaline/Norepinephrine) ELISA Kit
The NA/NE ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with NA/NE. During the reaction, NA/NE in the sample or standard competes with a fixed amount of NA/NE on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to NA/NE. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NA/NE in the samples is then determined by comparing the O.D. of the samples to the standard curve.<
Detected Target name: NA/NE
Abbreviation : Norepinephrine, NE, noradrenaline, NA, NAd, norad, 4,5-β-trihydroxyphenethylamine
Format : 96 rx
Reactivity : Universal (All Species expexted)
ELISA configuration : Competitive
Sensitivity : 0,188
Detection Range : 0.313-20 ng/mL
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