DA (Dopamine) ELISA Kit - E-EL-0046

DA (Dopamine) ELISA Kit

The DA ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with DA. During the reaction, DA in the sample or standard competes with a fixed amount of DA on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to DA. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DA in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: DA

Abbreviation : 2-(3,4-dihydroxyphenyl)ethylamine,3,4-dihydroxyphenethylamine,3-hydroxytyramine, DA, Intropin, Revivan, Oxytyramine

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 18,75

Detection Range : 31.25-2000 pg/mL

More information about this DA (Dopamine) ELISA Kit from Elabscience