VE (Vitamin E) ELISA Kit – E-EL-0018

VE (Vitamin E) ELISA Kit

The VE ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VE. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for VE and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VE. You can calculate the concentration of VE in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: VE

Abbreviation : Tocopherol

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,938

Detection Range : 1.563-100 μg/ml

More information about this VE (Vitamin E) ELISA Kit from Elabscience