VD3 (Vitamin D3) ELISA Kit – E-EL-0014

VD3 (Vitamin D3) ELISA Kit

The VD3 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to VD3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for VD3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain VD3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of VD3. You can calculate the concentration of VD3 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: VD3

Abbreviation : Cholecalciferol,toxiferol,vitamin D3

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 0,938

Detection Range : 1.563-100 ng/mL

More information about this VD3 (Vitamin D3) ELISA Kit from Elabscience