ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit – E-EL-0033

ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit

The ST/5-HT ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with ST/5-HT. During the reaction, ST/5-HT in the sample or standard competes with a fixed amount of ST/5-HT on the solid phase supporterfor sites on the Biotinylated Detection Ab specific to ST/5-HT. Excess conjugate and unbound sample or standard are washed from the plate, andAvidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ST/5-HT in the samples is then determined by comparing the O.D. of the samples to the standard curve.<

Detected Target name: ST/5-HT

Abbreviation : 5HT, Enteramine, Thrombocytin, Thrombotonin, 3-(β-Aminoethyl)-5-hydroxyindole

Uniprot ID :

Format : 96 rx

Reactivity : Universal (All Species expexted)

ELISA configuration : Competitive

Sensitivity : 9,375

Detection Range : 15.625-1000 ng/mL

More information about this ST/5-HT (Serotonin/5-Hydroxytryptamine) ELISA Kit from Elabscience