OT (Oxytocin) ELISA Kit
The OT ELISA kit is configured as a competitive assay. The microtiter plate provided in this kit has been pre-coated with OT. During the reaction, Universal OT in the sample or standard competes with a fixed amount of OT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal OT. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal OT in the samples is then determined by comparing the O.D. of the samples to the standard curve.<
Detected Target name: OT
Abbreviation : OXT, Pitocin
Format : 96 rx
Reactivity : Universal (All Species expexted)
ELISA configuration : Competitive
Sensitivity : 9,375
Detection Range : 15.625-1000 pg/mL
More information about this OT (Oxytocin) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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Reactivity | Universal |
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