TransScript® -Uni One-Step gDNA Removal and cDNA Synthesis SuperMix (with oligos) – AU311

TransScript® -Uni One-Step gDNA Removal and cDNA Synthesis SuperMix

TransScript®-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature (42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds.

Advantages of TransScript®-Uni RT

• Broad range reaction temperature (42°C-65°C).
• Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
• The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
• cDNA up to 20 kb.

Applications

• Multiple copy and low copy gene detection
• GC-rich or complex secondary structure RNA template
• cDNA library construction, primer extension, 3’ and 5’ RACE

Manual and datasheet

AU311 – TransScript Uni

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