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TransScript® -Uni One-Step gDNA Removal and cDNA Synthesis SuperMix (with oligos) – AU311

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TransScript® -Uni One-Step gDNA Removal and cDNA Synthesis SuperMix

TransScript®-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature (42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds.


Advantages of TransScript®-Uni RT

  • Broad range reaction temperature (42°C-65°C).
  • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination.
  • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR.
  • cDNA up to 20 kb.

Applications

  • Multiple copy and low copy gene detection
  • GC-rich or complex secondary structure RNA template
  • cDNA library construction, primer extension, 3’ and 5’ RACE

 

TGAU311 contentOligodT TGAU311 gel

 


Manual and datasheet

AU311 – TransScript Uni

 


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Additional information

Format

50 rx (20ul), 100 rx (20ul)

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