EasyScript® One-Step RT-PCR SuperMix (+dye) – AE411

EasyScript® One-Step RT-PCR SuperMix (+dye)

EasyScript® One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. EasyScript RT and TransTaq HiFi DNA Polymerase are used in the kit.

Advantages

• High efficiency cDNA synthesis with EasyScript One-Step Enzyme Mix and 2x One-Step Reaction Mix, followed by PCR amplification with resulting cDNA.
• Simple procedure helps to minimize contamination.
• Amplification product contains base “A” at 3’ end. Purified product is well suitable for cloning into pEASY-T vectors.
• Amplified fragment up to 4 kb.
• RNase inhibitor included in the mix.

Manual and Datasheet

AE411 EasyScript® One-Step RT-PCR SuperMix