EasyScript® One-Step RT-PCR SuperMix (+dye) – AE411
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EasyScript® One-Step RT-PCR SuperMix (+dye)
EasyScript® One-Step RT-PCR combines the first-strand cDNA synthesis with PCR in the same tube to simplify reaction setup and reduce the possibility of contamination. Only gene-specific primers can be used for One-Step RT-PCR. EasyScript RT and TransTaq HiFi DNA Polymerase are used in the kit.
- High efficiency cDNA synthesis with EasyScript One-Step Enzyme Mix and 2x One-Step Reaction Mix, followed by PCR amplification with resulting cDNA.
- Simple procedure helps to minimize contamination.
- Amplification product contains base “A” at 3’ end. Purified product is well suitable for cloning into pEASY-T vectors.
- Amplified fragment up to 4 kb.
- RNase inhibitor included in the mix.
Manual and Datasheet