Rat WNT10B ( Wingless-Type MMTV Integration Site Family, Member 10B) ELISA Kit – E-EL-R2578

Rat WNT10B ( Wingless-Type MMTV Integration Site Family, Member 10B) ELISA Kit

The WNT10B ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat WNT10B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat WNT10B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat WNT10B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat WNT10B. You can calculate the concentration of Rat WNT10B in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Wingless-Type MMTV Integration Site Family, Member 10B

Abbreviation : WNT10B

Uniprot ID : A0A0G2K6Q8

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.188 ng/ml

Detection Range : 0.313 – 20 ng/ml

Manual & Datasheet

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