Rat TrxR1 (Thioredoxin Reductase 1) ELISA Kit
The TrxR1 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TrxR1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TrxR1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TrxR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TrxR1. You can calculate the concentration of TrxR1 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Thioredoxin Reductase 1
Abbreviation : TrxR1
Uniprot ID : O89049
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 0.094 ng/ml
Detection Range : 0.156 – 10 ng/ml
More information about this Rat TrxR1 (Thioredoxin Reductase 1) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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