Rat TPO-Ab (Thrombopoietin Antibody) ELISA Kit – E-EL-R0961

Rat TPO-Ab (Thrombopoietin Antibody) ELISA Kit

The TPO-Ab ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antigen specific to Rat TPO-Ab. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antigen. Then a biotinylated detection antigen specific for Rat TPO-Ab and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TPO-Ab, biotinylated detection antigen and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TPO-Ab. You can calculate the concentration of Rat TPO-Ab in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Anti-Thrombopoietin

Abbreviation : TPO-Ab

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 3.75 ng/ml

Detection Range : 6.25 – 400 ng/ml

Manual & Datasheet

More information about this Rat TPO-Ab (Thrombopoietin Antibody) ELISA Kit from Elabscience