Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit – E-EL-R1091

Rat TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit

The TNFRSF1A ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNFR1/TNFRSF1A. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TNFR1/TNFRSF1A and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNFR1/TNFRSF1A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TNFR1/TNFRSF1A. You can calculate the concentration of TNFR1/TNFRSF1A in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Tumor Necrosis Factor Receptor Superfamily, Member 1A

Abbreviation : TNFRSF1A

Uniprot ID : P22934

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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