Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit
The TNF-α ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TNF-α. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TNF-α. You can calculate the concentration of TNF-α in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Tumor Necrosis Factor Alpha
Abbreviation : TNF-α
Uniprot ID : P16599
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 46.875 pg/ml
Detection Range : 78.125 – 5000 pg/ml
More information about this Rat TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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