Rat TLR4 (Toll-Like Receptor 4) ELISA Kit
The TLR4 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TLR4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for TLR4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TLR4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of TLR4. You can calculate the concentration of TLR4 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Toll-Like Receptor 4
Abbreviation : TLR4
Uniprot ID : Q9QX05
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 0.188 ng/ml
Detection Range : 0.313 – 20 ng/ml
More information about this Rat TLR4 (Toll-Like Receptor 4) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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