Rat TAF4 (Tata Box Binding Protein Associated Factor 2) ELISA Kit – E-EL-R0942

Rat TAF4 (Tata Box Binding Protein Associated Factor 2) ELISA Kit

The TAF4 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TAF4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TAF4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TAF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TAF4. You can calculate the concentration of Rat TAF4 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Tata Box Binding Protein Associated Factor 2

Abbreviation : TAF4

Uniprot ID :

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 9.375 pg/ml

Detection Range : 15.625 – 1000 pg/ml

Manual & Datasheet

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