Rat SSFA2 (Sperm Specific Antigen 2) ELISA Kit
The SSFA2 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SSFA2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat SSFA2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SSFA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SSFA2. You can calculate the concentration of Rat SSFA2 in the samples by comparing the OD of the samples to the standard curve.
Detected Target name: Rat Sperm Specific Antigen 2
Abbreviation : SSFA2
Uniprot ID : D3ZLC3
Format : 96 rx
Reactivity : Rat
ELISA configuration : Sandwich
Sensitivity : 0.938 ng/ml
Detection Range : 1.563 – 100 ng/ml
More information about this Rat SSFA2 (Sperm Specific Antigen 2) ELISA Kit from Elabscience
Additional information
Format | 96 rx |
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