Rat SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit – E-EL-R0831

Rat SPD (Pulmonary Surfactant Associated Protein D) ELISA Kit

The SPD ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to SPD. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for SPD and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SPD, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of SPD. You can calculate the concentration of SPD in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Pulmonary Surfactant Associated Protein D

Abbreviation : SPD

Uniprot ID : P35248

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 46.875 pg/ml

Detection Range : 78.125 – 5000 pg/ml

Manual & Datasheet

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