Rat DDR1 Kinase / MCK10 / CD167 Protein (Fc tag) – PKSR030165

Recombinant Rat DDR1 Kinase / MCK10 / CD167 Protein (Fc tag)

Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway.

Synonym(s) : DDR1

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat DDR1 (Q6MG19) (Met1-Thr413) was expressed with the Fc region of human IgG1 at the C-terminus.

Predicted N-terminal amino acid : Asp 20

Molecular weight/mass : The recombinant rat DDR1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 635 amino acids and has a predicted molecular mass of 70.9 kDa. The apparent molecular mass of the protein is approximately 71 kDa in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the rat DDR1 (Q6MG19) (Met1-Thr413) was expressed with the Fc region of human IgG1 at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 95 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet