Rat Cripto / TDGF1 Protein (Fc tag) – PKSR030352

Recombinant Rat Cripto / TDGF1 Protein (Fc tag)

Cripto/TDGF1 is a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family. EGF-CFC family member proteins share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. Before gastrulation, Cripto is asymmetrically expressed in a proximal–distal gradient in the epiblast, and subsequently is expressed in the primitive streak and newly formed embryonic mesoderm. These proteins play key roles in intercellular signaling pathways during vertebrate embryogenesis. Mutations in Cripto/TDGF1 can cause autosomal visceral heterotaxy. Cripto/TDGF1 is involved in left-right asymmetric morphogenesis during organ development. Cripto signalling is essential for the conversion of a proximal–distal asymmetry into an orthogonal anterior–posterior axis. The mechanism of inhibitory effects of the Cripto includes both cancer cell apoptosis, activation of c-Jun-NH(2)-terminal kinase and p38 kinase signaling pathways and blocking of Akt phosphorylation. Thus, Cripto is a unique target, and Immunohistochemistry to Cripto could be of therapeutic value for human cancers.

Synonym(s) : FGFR-4

Species(s) : Rat

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the rat TDGF1 (XP_001056317.2) (Met 1-Cys 143) was fused with the Fc region of human IgG1 at the C-terminus.

Predicted N-terminal amino acid : Phe 17

Molecular weight/mass : The recombinant rat TDGF1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 368 amino acids and predicts a molecular mass of 41 kDa. The apparent molecular mass of the rat TDGF1/Fc monomer is approximately 45 kDa in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the rat TDGF1 (XP_001056317.2) (Met 1-Cys 143) was fused with the Fc region of human IgG1 at the C-terminus.

Formulation : Lyophilized from sterile PBS, pH 7.4

Purity : > 90 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet