Vimentin Protein – Prot-r-Vim

Vimentin Purified Protein : Vimentin is a member of the intermediate or 10nm family of proteins, which are abundant structural components of most eukaryotic cells. Intermediate filament proteins are divided into 5 classes, with class I and II including the epithelial keratins, class IV the neurofilaments and class V the nuclear lamins. Class III includes vimentin along with desmin, peripherin and glial fibrillary acidic protein (GFAP). Vimentin is found in most cell lines grown in tissue culture and in many cell types early in development. In adult animals vimentin is found in mesenchymal cells, lymphocytes, many kinds of glia, endothelial cells and many others.

Characteristics of Recombinant human Vimentin protein

A cDNA encoding full length human vimentin was inserted into a eukaryotic expression vector which adds an N-terminal in frame His-tag. This was transformed into E. coli and recombinant protein was purified in 6M urea using immobilized metal affinity chromatography. Purified protein was diluted to 1 mg/mL and is supplied in 6M urea.

Encor Vimentin Protein – Prot-r-Vim

Formats available : 50 ug, 100 ug

Datasheet

Link to Vimentin Protein – Prot-r-Vim on Encor’s website

References :

1. Franke, W. W., Schmid, E., Osborn, M. and Weber, K. Different intermediate-sized filaments distinguished by immunofluorescence microscopy. Proc. Natl. Acad. Sci. USA 75:5034–5038 (1978).

2. Muller, M., Bhattacharya, S. S., Moore, T., Prescott, Q., Wedig, T., Herrmann, H., Magin, T. M. Dominant cataract formation in association with a vimentin assembly disrupting mutation. Hum. Molec. Genet. 18:1052-1057 (2009).