Human IL13RA2 / IL13R Protein (His & Fc tag) – PKSH031658

Recombinant Human IL13RA2 / IL13R Protein (His & Fc tag)

Interleukin-13 receptor subunit alpha-2 (IL13RA2/IL-13RA2) is also known as also known as cluster of differentiation 213A2 (CD213A2), IL-13 receptor subunit alpha-2, IL-13R subunit alpha-2, and IL-13RA2. The IL13RA2 is often overexpressed in brain tumors, making Il13ra2 one of the vaccine targets for immunotherapy of glioma. IL13RA2/IL-13RA2 is a cancer-associated receptor that is present in greater than 80% of High Grade Astrocytomas (HGA) and has recently been recognized as a cytokine that predisposes breast cancer cells to metastasize. Expression of IL13Rα2 was rapidly lost from the surface of transduced cells grown in culture. The loss appeared to be related to ligands present in fetal bovine serum in the medium. None of the malignant glioma cell lines cultivated in vitro and tested to date exhibited the IL13Rα2 receptor. A recombinant virus (R5111) enters cells via its interaction with the IL13Rα2 receptor in a manner that cannot be differentiated from the interaction of wild-type virus with its receptors.

Synonym(s) : CD213A2 , IL-13R , IL13BP

Species(s) : Human

Protein Expression Host : Human Cells

Protein Construction : A DNA sequence encoding the extracellular domain of human IL13Rα2 (NP_000631.1) (Met 1-Leu 342) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Predicted N-terminal amino acid : Asp 27

Molecular weight/mass : The recombinant human IL13Rα2/Fc is a disulfide-linked homodimeric protein after proteolytic removal of the signal peptide. The reduced monomer consists of 563 amino acids and predicts a molecular mass of 65 kDa. As a result of glycosylation, the rh IL13Rα2/Fc monomer migrates as an approximately 90-100 kDa band in SDS-PAGE under reducing conditions.

Protein Construction : A DNA sequence encoding the extracellular domain of human IL13Rα2 (NP_000631.1) (Met 1-Leu 342) was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Formulation : Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5

Purity : > 75 % as determined by SDS-PAGE

Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method

Stability : Samples are stable for up to twelve months from date of receipt at -70°C

Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Reconstitution : Please refer to the printed manual for detailed information.

Manual & Datasheet