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Human PAPPA2 / Pappalysin 2 Protein (His tag) – PKSH031546

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Recombinant Human PAPPA2 / Pappalysin 2 Protein (His tag)

Pappalysin-2/PAPP-A2 is the second member of the pappalysin family of metzincin superfamily, of which PAPP-A is the first member. There is no homology between the prepro-peptides of PAPP-A and PAPP-A2, but 46% of the residues of mature PAPP-A are also present in mature PAPP-A2. PAPP-A specifically cleaves insulin-like growth factor-binding protein(IGFBP)-4, one of six known modulators of IGF-I and –II, whereas PAPP-A2 specifically cleaved IGFBP-5 at one site, between Ser-143 and Lys-144. In contrast to the cleavage of IGFBP-4 by PAPP-A that strictly requires the presence of IGF, the cleavage of IGFBP-5 by PAPP-A2 was IGF-independent. Recent data firmly establish PAPP-A and IGFBP-4 as an important functional pair in several systems. Because of its close relationship with PAPP-A, both structurally and functionally, PAPP-A2 is a likely candidate for IGFBP-5 proteinase in many tissues and conditioned media where IGFBP-5 proteolysis has been reported.


Synonym(s) : RP4-774I24.1 , PAPP-A2 , PAPPE , PLAC3


Species(s) : Human


Protein Expression Host : Human Cells


Protein Construction : A DNA sequence encoding the human PAPPA2 mature form (NP_064714.2) corresponding to amino acid (Ser 234-Cys 1396) was expressed, with a carboxy-terminal polyhistidine tag.


Predicted N-terminal amino acid : Ser 234


Molecular weight/mass : The secreted recombinant human PAPPA2 consists of 1174 amino acids with the predicted molecular mass of 131 kDa. As a result of glycosylation, rhPAPPA2 migrates as an approximately 170-180 kDa band in SDS-PAGE under reducing conditions.


Protein Construction : A DNA sequence encoding the human PAPPA2 mature form (NP_064714.2) corresponding to amino acid (Ser 234-Cys 1396) was expressed, with a carboxy-terminal polyhistidine tag.


Formulation : Lyophilized from sterile PBS, pH 7.4


Purity : > 90 % as determined by SDS-PAGE


Endotoxin level : < 1.0 EU per μg of the protein as determined by the LAL method


Stability : Samples are stable for up to twelve months from date of receipt at -70°C


Shipping conditions : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.


Reconstitution : Please refer to the printed manual for detailed information.


Manual & Datasheet


Additional information

Format

20 ug, 50 ug

Species

Human

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