Rat C4 (Complement Component 4) ELISA Kit – E-EL-R0248

Rat C4 (Complement Component 4) ELISA Kit

The C4 ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for C4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of C4. You can calculate the concentration of C4 in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Complement Component 4

Abbreviation : C4

Uniprot ID : P08649

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 1.875 ng/ml

Detection Range : 3.125 – 200 ng/ml

Manual & Datasheet

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