Rat C1R (Complement Component 1Q Receptor) ELISA Kit – E-EL-R0249

Rat C1R (Complement Component 1Q Receptor) ELISA Kit

The C1R ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat C1R. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat C1R and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat C1R, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat C1R. You can calculate the concentration of Rat C1R in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Complement Component 1Q Receptor

Abbreviation : C1R

Uniprot ID : Q9ET61

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 0.938 ng/ml

Detection Range : 1.563 – 100 ng/ml

Manual & Datasheet

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