Rat C1INH (Complement 1 Inhibitor) ELISA Kit – E-EL-R0246

Rat C1INH (Complement 1 Inhibitor) ELISA Kit

The C1INH ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to C1INH. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for C1INH and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain C1INH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of C1INH. You can calculate the concentration of C1INH in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Complement 1 Inhibitor

Abbreviation : C1INH

Uniprot ID : Q6P734

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 4.688 ng/ml

Detection Range : 7.813 – 500 ng/ml

Manual & Datasheet

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