Rat BTC (Betacellulin) ELISA Kit – E-EL-R0101

Rat BTC (Betacellulin) ELISA Kit

The BTC ELISA kit is configured as a Sandwich antibody assay. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to BTC. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for BTC and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain BTC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of BTC. You can calculate the concentration of BTC in the samples by comparing the OD of the samples to the standard curve.

Detected Target name: Rat Betacellulin

Abbreviation : BTC

Uniprot ID : Q9JJM4

Format : 96 rx

Reactivity : Rat

ELISA configuration : Sandwich

Sensitivity : 5.625 pg/ml

Detection Range : 9.375 – 600 pg/ml

Manual & Datasheet

More information about this Rat BTC (Betacellulin) ELISA Kit from Elabscience